Render 3D animations as 2D sprites in 4, 8, or 16 directions.
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Set up camera and lighting to your liking. For a proper isometric perspective set the camera's x rotation to 60 degrees. Make sure the camera's z is set to 0 before you hit render.
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Make sure both the lighting and camera are selected. You can hit the rotate button to make sure the camera is rotating around the object properly.
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Hit render.