Comparative genomics-assisted motif discovery
This document provides detailed information on the configuration parameters used in the project. The configuration file follows a schema defined by Cerberus, ensuring that all inputs meet the specified criteria. Below are the parameters and their validation rules.
The entrez_parameters section configures parameters related to Entrez queries.
- request_limit (integer, required): the maximum number of requests allowed. Must be at least 1.
- sleep_time (number, required): the time (in seconds) to wait between requests. Must be a non-negative number.
- email (string, required): a valid email address used for Entrez queries. Must match the regex pattern for email addresses.
- api_key (string, optional): the API key for Entrez. Can be
null.
The blast_parameters section configures parameters related to BLAST searches.
- db (string, optional): the database to search against. Can be
null. - tax_IDs (list, optional): a list of taxonomy IDs to include in the search. Can be
null. - e_value (number, optional): the E-value threshold for the BLAST search. Can be
null. - query_coverage (number, optional): the query coverage percentage. Can be
null. - max_hits (integer, optional): the maximum number of hits to return. Must be at least 1 if specified. Can be
null.
The sequences_parameters section configures parameters related to sequence processing.
- max_intergenic_size (number, required): the maximum intergenic size. Cannot be
null. - min_intergenic_size (number, required): the minimum intergenic size. Cannot be
null. - upstream_size_region (number, required): the size of the upstream region. Cannot be
null. - downstream_size_region (number, required): the size of the downstream region. Cannot be
null. - max_sequence_length (integer, optional): the maximum sequence length. Can be
null.
- max_identity (float, required): the maximum identity threshold. Must be between 0.0 and 1.0 inclusive. Cannot be
null.
The meme_parameters section configures parameters for MEME motif discovery.
- mod (string, required): the model to use. Cannot be
null. - nmotifs (integer, required): the number of motifs to find. Cannot be
null. - minw (integer, required): the minimum motif width. Must be at least 0. Cannot be
null. - maxw (integer, required): the maximum motif width. Cannot be
null. - revcomp (boolean, required): whether to search both strands. Cannot be
null. - pal (boolean, required): whether to search for palindromic motifs. Cannot be
null.
- run_only_meme (boolean, required): flag to indicate if only MEME should be run. Cannot be
null.
The output_parameters section configures parameters related to output directories.
- folder_name (string, required): the name of the output folder. Cannot be
null. - folder_rerun_meme (string, optional): the folder name for rerunning MEME. Can be
null.
- input_records (list, required): a list of input records. Must contain at least one record. Cannot be
null.
Ensure you have access to the command line interface on your operating system. This could be Terminal on macOS or Linux, or Command Prompt/PowerShell on Windows.
Change your current directory to the root directory of the project where the main script is located. You can use the cd command followed by the path to the project directory. For example:
cd path/to/your/projectTo run the main pipeline, use the Python interpreter followed by the script name and the required root directory argument. The root directory is the main directory where the pipeline will operate. The command structure is as follows:
python main.py <root_directory>If your root directory is located at /data/project_root, the command would be:
python main.py /data/project_root