EDePasquale / DoubletDecon

A tool for removing doublets from single-cell RNA-seq data

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Opening DoubletDecon Shiny app

abigailelliot26 opened this issue · comments

Hi,

I'm having trouble opening DoubletDecon in Shiny. I've tried both of your suggestions from below. When I try downloading and clicking on DoubletDecon.app I get this error message : "“DoubletDecon.app” is damaged and can’t be opened. You should move it to the Trash." When I try using runGist as above I get the following error: "Error: shinyjs: extendShinyjs: functions argument must be provided".

Any suggestions? (I'm on a Mac)

best wishes,

Abigail


On the project page for DoubletDecon (https://github.com/EDePasquale/DoubletDecon) select "clone or download" and then "download ZIP". When this is finished, decompress the file. Within this folder, doublet clicking on the "DoubletDecon" application file should open R for you as well as a web browser with the DoubletDecon user interface. I use this because it gives me a DoubletDecon icon I can add to my hot bar for easy access.

I'm unsure if this works for Windows machines, but if it doesn't or if you don't want the clickable button run the following line in R or R Studio:

shiny::runGist('a81cdc2aea5742c08e5fc3fa66d47698', launch.browser=TRUE)

I am working of writing a full protocol for running DoubletDecon both from the command line and the user interface, with pre-processing and analysis steps, which will hopefully be submitted soon!

Please let me know if you have any more questions.

Thanks,
Erica

Originally posted by @EDePasquale in #14 (comment)

Very weird! I made a change to the the extendShinyjs() function in the backend according to https://stackoverflow.com/questions/63860952/print-shiny-app-screen-not-working-error-shinyjs-extendshinyjs-functions-ar. I added 'function = c("diableTab")' and it appears to load for me without immediately crashing. I'm not sure what change occurred in that package but please let me know if it is still giving you trouble.

Thanks,
Erica