EDePasquale / DoubletDecon

A tool for removing doublets from single-cell RNA-seq data

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the results of Demuxlet dataset

JuanruMaryGuo opened this issue · comments

Hi, I'm trying to run DoubletDecon using Demuxlet dataset by running 20 times. However, I haven't gotten a result with high accuracy, I'm wondering if there are some errors in my code. Would you please share your code for reference?

By the way, I failed to run the code with the rho bigger than or equal to 1 and finally made it to be 0.61 after consulting other issues in Github. Might it cause the problem?

Thank you in advance!

Hi,

Are you doing each run with the exact same parameters?

Yes, I just ran the same code 20 times and all of the parameters are the same in the example except rho = 0.61.