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DavisLaboratory / standR

Spatial transcriptomics analyses and decoding in R

Home Page:https://davislaboratory.github.io/standR/

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`norm.factors` not being transferred from `SpatialExperiment` to `DGEList`

jaredmcollette opened this issue · comments

commented

When using TMM normalisation on a spatial experiment object using geomxNorm(), the norm.factors are stored in the metadata.

Later, when using SE2DGEList() to convert the spatial experiment object to a dgeList, the norm.factors are not automatically carried over to the dge, and I have to manually transfer over the norm.factors:

dge$samples$norm.factors <- metadata(spe_tmm)$norm.factor

It would be helpful to have this process automated

commented

Hi @jaredmcollette , thanks for the suggestion. The SE2DGEList is from the edgeR package, hence wouldn't aware any of the metadata we stored in the spe. I'll probably write a new function to replace SE2DGEList before the next release to incorporate the suggested ability.