spyros-lytras / ancient_bat_OAS1

This repository contains online supplementary materials for the paper: Resurrection of 2′-5′-oligoadenylate synthetase 1 (OAS1) from the ancestor of modern horseshoe bats blocks SARS-CoV-2 replication, published in PLOS Biology (Lytras et al., 2023).

Sequences & Alignments

• Coding sequence alignment corresponding to the protein alignment used for the ancestral state reconstruction

• Ancestrally reconstructed protein sequences for the Rhinolophoidea common ancestor OAS (RhinoCA and RhinoCA-T70

• cDNA constructs with flanking SfiI sites synthesised to be subcloned into lentiviral vectors for cell line expression

• Coding sequence alignment used for the selection analysis

• Full protein sequence alignment of all Chiroptera OAS1 proteins

Phylogenetic trees

• Guide species tree for bat species used in the analysis (retrieved from TimeTree)

• Ancestral state reconstruction inferred bat OAS1 phylogeny

Structural predictions

• RhinoCA OAS1 structural prediction

• Rhinolophus ferrumequinum OAS1 structural prediction

• Pteropus alecto OAS1 structural prediction

Tabular data

• Sequence metadata

• Selection analysis and per site entropy results

• Protein domain coordinates