Add counts of different alleles at each site in each sample in a reference free alignment
gtonkinhill opened this issue · comments
Gerry Tonkin-Hill commented
Simon Harris commented
SKA doesn't have that information at that stage, but I could output it from ska fastq and provide a way to look up allele frequencies based on a set of split kmers. Would that work?
Gerry Tonkin-Hill commented
Yeah that would be great! It would be very helpful for any within-host mixed sample analysis.
Simon Harris commented
I've added the option to output allele counts during ska fastq and also ska humanise to output kmers in a human readable format (with the middle bases of each sample). You should be able to use the first column from that to find your allele frequencies in the allele count files.