QC ALERT
drosop opened this issue · comments
Dear MiXCR team,
I have run the mouse BCR sequencing using Takara SMARTer® Mouse BCR IgG H/K/L Profiling Kit and did analysis using the MiXCR preset mixcr analyze takara-mouse-rna-bcr-smarter using MiXCR v4.5.0. Also, There was very low adaptor content, so I didnt do trimming before.
In the output, I have alerts for :
Successfully aligned reads: 97.67% [OK]
Off target (non TCR/IG) reads: 1.8% [OK]
Reads with no V or J hits: 0.49% [OK]
Overlapped paired-end reads: 32.07% [ALERT]
Reads used in clonotypes: 19.68% [ALERT]
Alignments that do not cover VDJRegion: 68.03% [ALERT]
Alignments dropped due to low sequence quality: 0.0% [OK]
Clones dropped in post-filtering: 0.0% [OK]
Alignments dropped in clones post-filtering: 0.0% [OK]
However, the alignment rate is good. Is this report fine? What can be the reason for low number of reads used in clonotypes or VDJ regions?
Thank you,