_2022_Norrild_GMMA_TRX
Scripts and output from "Increasing protein stability by inferring substitution effects from high-throughput experiments" by Rasmus K. Norrild, Kristoffer E. Johansson, Charlotte O’Shea, Jens Preben Morth, Kresten Lindorff-Larsen and Jakob R. Winther
Directories
- variant_call_scripts: Code mentioned in the article used for processing FASTQ files
- data: Raw data from the CPOP screen and computational methods including plots
- GMMA: Software, input and output files. The analysis is implemented in R and split into 5 scripts (gmma01-gmma05), see run.sh. The excel file trx_gmma.xlsx contains all 3 output files residue.txt, subst.csv and mutant.csv.
- nano_dsf: Data and notebook for stability measurements
Sequencing reads are available at https://erda.ku.dk/archives/66b4989dbfdc6374ba673594df195465/published-archive.html
