jianhong / ATACseqQC

ATAC-seq Quality Control

Home Page:https://jianhong.github.io/ATACseqQC/articles/ATACseqQC.html

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What does the x axis mean for Nucleosome Free Regions (NFR) score plot?

hyjforesight opened this issue · comments

Hello ATACseqQC,
Thanks for developing this amazing package.
Could you please tell me what's the meaning of the x axis of Nucleosome Free Regions (NFR) score plot? It says 'the average signals of 400 bp window as X-axis', but the title of NFR score plot shows 'NFRscore for 200bp flanking TSSs'.
image
Thanks!
Best,
YJ

Thank you for your good words. The 200bp in the plot title means upstream 200bp + downstream 200bp. Sorry for the confusion.

Hello @jianhong
Thanks for the response. Now I get it. Sorry, I'm also confused about the meaning of the values of x-axis. Are they the values of log2 mean coverage, not the distance (bp) to the TSS?

Yes, they are log2 mean coverage.