jianhong / ATACseqQC

ATAC-seq Quality Control

Home Page:https://jianhong.github.io/ATACseqQC/articles/ATACseqQC.html

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How to normalize cut-site probability between samples

kshimizu64928 opened this issue · comments

Hello,

I want to compare cut-site probability between samples.
However, I found that the backgrounds of cut-site probabilities of each sample were linearly correlated with mapped read counts of each sample.
Background was calculated as mean cut-site probability sufficiently far from motif (+5kb ~ +10kb).
Do you have any ideas to normalize cut-site probability between samples?

Thanks in advance,