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griffithlab / rnaseq_tutorial

Informatics for RNA-seq: A web resource for analysis on the cloud. Educational tutorials and working pipelines for RNA-seq analysis including an introduction to: cloud computing, critical file formats, reference genomes, gene annotation, expression, differential expression, alternative splicing, data visualization, and interpretation.

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link broken - adapter trim

frankbioinfo opened this issue · comments

commented

Hello Griffith Lab team members,

Thank you for providing such a nice explanation and tutorial on RNA-Seq analysis.

I was going over the adapter trimming tutorial, and found that this link

wget http://genomedata.org/rnaseq-tutorial/illumina_multiplex.fa

is broken , could you please help in fixing it.

Thank you.

commented

Sorry for the inconvenience.

We switched to using a dedicated server for this recently. The whole thing seems to be unreachable at present. Investigating now...

commented

That was my bad. I had an entry in the fstab file whose format used to work but was not compatible with ubuntu LTS 16.04 for some reason. When I made an AMI of the genomedata web server (in case we ever need to re-initiate from scratch), that triggered a reboot, and the fstab error prevented successful boot, and made the instance unusable. I've re-created the instance (and AMI) with the fstab entry fixed, re-associated the elastic IP, and the genomedata server is now back up. Apologies for this inconvenience.

commented

Thank you Dr @malachig and Dr @obigriffith for your prompt response. Appreciate it.