[User Story] Verify results by mapping
pbiology opened this issue · comments
Anders Lind commented
Need
As a clinician I want to be able to "verify" if the OTUs the classifiers have identified are correct so I can feel certain in my interpretation.
Suggested approach
- A click button to run post-processing in a separate repository to validate the interesting species.
- KrakenTools will be used to extract reads from Kraken2 report. Custom scripts will be needed to extract reads from reports generated by other classifiers.
- Run BLAST separately for the species of interest.
- Mapping: Map the extracted reads against the target genome and the human genome.
Can be closed when
- We've decided on what a good approach to this is
Blockers
No response
Anything else?
We have to figure out how this should be approached. There are multiple things to take into consideration.
- If the visualisation tool should launch new compute jobs, this needs to be agnostic to infrastructure as this is a national tool.
- If the visualiser starts performing calculations, this might push it into the frame of being a IVDR classified product and would have to comply to those rules, which are extensive when it comes to products with a GUI.
- Could the pipeline perform these alignments, and if so, on which OTUs? Can't be all of them.