epinatel

Bacterial_RNAseq

This bacterial-RNAseq analysis pipeline is based on four main steps: (a) reads alignment and quality filtering; (b) alignment visualization; (c) gene counts production; and (d) gene counts normalization and differential expression analysis. We embedded in the analysis workflow five checkpoints regarding: (1) the evalua- tion of sequencing performances; (2) the calculation of genome alignment levels; (3) the estimation of the quality of the library (adapter presence, rRNA removal, and strand specificity); (4) the assessment of global gene coverage; and (5) the control of sample reproducibility