sevenbridges: R Package for Seven Bridges Platform from API client to CWL generator
Bioconductor-Stable | Bioconductor-Devel
Table of Contents
Events
Features
Installation
Tutorial
IDE docker image
Issues report
Q and A
Events
- September 12 - 14, 2016 Probabilistic Modeling in Genomics @ Oxford University, UK (poster)
- May 27 - 29, 2016 The 9th China-R Conference 2016 @ Renmin University, Beijng, China. (talk)
- June 24 - 26, 2016 BioC 2016: Where Software and Biology Connect @ Stanford University, Stanford, CA (workshop)
- June 27 - 30, 2016 The R User Conference 2016 @ Stanford University, Stanford, CA (talk)
- (Past) NCI Cancer Genomics Cloud Hackathon Tutorials @ Boston 2016 [html] [R markdown] [R script]
Features
- Complete API R client with user friendly API in object-oriented fashion with user friendly printing, support operation on users, billing, project, file, app, task etc, short example.
## get project by pattern matching name
p = a$project("demo")
## get exact project by id
p = a$project(id = "tengfei/demo")
## detele files from a project
p$file("sample.tz")$delete()
## upload fies from a folder to a project with metadata
p$upload("folder_path", metadata = list(platform = "Illumina"))
- Task monitoring hook allow you to add hook function to task status when you monitor a task, for example, when task is finished sent you email or download all files.
setTaskHook("completed", function(){
tsk$download("~/Downloads")
})
tsk$monitor()
- Task batching by item and metadata
## batch by items
(tsk <- p$task_add(name = "RNA DE report new batch 2",
description = "RNA DE analysis report",
app = rna.app$id,
batch = batch(input = "bamfiles"),
inputs = list(bamfiles = bamfiles.in,
design = design.in,
gtffile = gtf.in)))
## batch by metadata, input files has to have metadata fields specified
(tsk <- p$task_add(name = "RNA DE report new batch 3",
description = "RNA DE analysis report",
app = rna.app$id,
batch = batch(input = "fastq",
c("metadata.sample_id",
"metadata.library_id")),
inputs = list(bamfiles = bamfiles.in,
design = design.in,
gtffile = gtf.in)))
-
Cross platform support from Seven Bridges, such as NCI Cancer genomics cloud or Seven bridges on google and AWS, you can use it.
-
Authentication management for multiple platforms/users via config file.
## standard
a = Auth(token = "fake_token", url = "api_url")
## OR from config file, multiple platform/user support
a = Auth(username = "tengfei", platform = "cgc")
- CWL Tool interface, you can directly describe your tool in R, export to JSON/YAML, or add it to your online project. This package defines a complete set of CWL object. So you can describe tools like this
library(readr)
fd <- fileDef(name = "runif.R",
content = read_file(fl))
rbx <- Tool(id = "runif",
label = "runif",
hints = requirements(docker(pull = "rocker/r-base"),
cpu(1), mem(2000)),
requirements = requirements(fd),
baseCommand = "Rscript runif.R",
stdout = "output.txt",
inputs = list(input(id = "number",
type = "integer",
position = 1),
input(id = "min",
type = "float",
position = 2),
input(id = "max",
type = "float",
position = 3)),
outputs = output(id = "random", glob = "output.txt"))
## output cwl JSON
rbx$toJSON(pretty = TRUE)
## output cwl YAML
rbx$toYAML()
- Utilities for Tool and Flow, for example
# converting a SBG CWL json file
library(sevenbridges)
t1 = system.file("extdata/app", "tool_star.json", package = "sevenbridges")
## convert json file into a Tool object
t1 = convert_app(t1)
# shows all input matrix
t1$input_matrix()
Installation
[Bioconductor: Stable]
For most users, I will recommend this installation, it's most stable. The current release of Bioconductor is version 3.3; it works with R version 3.3.0. Users of older R and Bioconductor versions must update their installation to take advantage of new features. If you don't want to update your R, please install from github directly as introduced in next section. It's quite easy.
Now check your R version
R.version.string
If you are not running latest R, first install R 3.3 following instruction here, after you successfully installed
R 3.3, if you are using Rstudio, please close and restart Rstudio, it will detect the
new install. Then install sevenbridges
package.
source("http://bioconductor.org/biocLite.R")
biocLite("sevenbridges")
[Latest]
You can always install the latest development version of the package from GitHub, it's always the most latest version, fresh new, with all new features and hot fixes, we push to bioconductor branch (release/devel) regularly.
If you don't have devtools
This require you have devtools
package, install it from CRAN if you don't have it
install.packages("devtools")
You may got an error and need system dependecies sometimes for curl and ssl, for example, in ubuntu you probably need to do this first in order to install devtools
and in order to build vigenttes (you need pandoc)
apt-get update
apt-get install libcurl4-gnutls-dev libssl-dev pandoc pandoc-citeproc
If devtools is already installed
Now install latest version from github for sevenbridges
source("http://bioconductor.org/biocLite.R")
biocLite(c("readr", "BiocStyle"))
library(devtools)
install_github("sbg/sevenbridges-r", build_vignettes=TRUE,
repos=BiocInstaller::biocinstallRepos(),
dependencies=TRUE)
If you have trouble with pandoc and don't want to install pandoc, set build_vignettes = FALSE
to avoid vignettes build,
[Bioconductor: Devel]
Install from bioconductor devel
branch if you are developing tools in devel branch or if you are users who use devel version for R and Bioconductor. You need to install R-devel first, please follow the instruction "Using the Devel Version of Bioconductor". After upgrade of R. This is kind of tricky and hard, if you just want to try latest feature, please install directly from github as next installation instruction.
source("http://bioconductor.org/biocLite.R")
useDevel(devel = TRUE)
biocLite("sevenbridges")
To load the package in R, simply call
library("sevenbridges")
Tutorials
We have 3 different version, github (latest), Bioconductor stable and devel. Only github version is provided below for latest docs. For other version, please visit Bioconductor homepage (link provide at the top). Tutorials below is auto-generated at 8:00pm everyday with github version.
- Complete Guide for API R Client [html] [R script]
- Tutorial: use R for Cancer Genomics Cloud [html] [R script]
- Creating Your Docker Container and Command Line Interface [html] [R script]
- Describe CWL Tools/Workflows in R and Execution [html] [R script]
- IDE container: Rstudio and Shiny server and more [html] [R script]
- Find Data on CGC via Data Exploerer, SPARQL and Data API [html] [R script]
Launch Rstudio Server and Shiny Server with sevenbridges IDE docker container
docker run -d -p 8787:8787 -p 3838:3838 --name rstudio_shiny_server tengfei/sevenbridges
To mount file system you need to use --privileged
with fuse.
docker run --privileged -d -p 8787:8787 -p 3838:3838 --name rstudio_shiny_server tengfei/sevenbridges
check out the ip from docker machine if you are on mac os.
docker-machine ip default
In your browser, http://<url>:8787/
for Rstudio server, for example, if 192.168.99.100 is what returned, visit http://192.168.99.100:8787/
for Rstudio.
For shiny server, per user app is hosted http://<url>:3838/users/<username of rstudio>/<app_dir>
for Shiny server, for example for user rstudio
(a default user) and some app called 01_hello
, it will be http://<url>:3838/users/rstudio/01_hello/
. To develop your shiny app as Rstudio user, you can login your rstudio server, and create a fold at home folder called ~/ShinyApps
and develop shiny apps under that folder, for example, you can create an app called 02_text
at ~/ShinyApps/02_text/
.
Login your rstudio at http://<url>:8787
, then try to copy some example over to your home folder
dir.create("~/ShinyApps")
file.copy("/usr/local/lib/R/site-library/shiny/examples/01_hello/", "~/ShinyApps/", recursive = TRUE)
If you are login as username 'rstudio', then visit http://192.168.99.100:3838/rstudio/01_hello
you should be able to see the hello example.
Note: Generic shiny app can also be hosted http://<url>:3838/
or for particular app, http://<url>:3838/<app_dir>
and inside the docker container, it's hosted under /srv/shiny-server/
Issue report
All feedback are welcomed! Please file bug/issue/request on the issue page here on github, we will try to respond asap.
Q&A
To ask a question about this package, the best place will be Bioconductor support website, go visit support website, and tag your post with package name sevenbridges.
-
Q: Does this package support older API which is not cwl compatible?
A: No it only supports API v2 +, for older version, please check sbgr package, but please note that the old API or project type will be deprecated. -
Q: Which version of CWL (common workflow language) supported now?
A: Draft 2, progress on draft 3. -
Q: Is there a python binding for the API?
A: Yes, official python client is here. And lots python recipes are now here -
Q: Why I always get warning message when I use API R client?
A: It only exists in Rstudio, potentially a bug in Rstudio. To ignore it useoptions(warn = -1)
-
Q: I still have some problem A: Please try use the latest package on github, at least update to your package on Bioconductor, that usually solved the most recent bugs.
© Seven Bridges Genomics 2012 - 2016. Licensed under the MIT license.