Files and source code used to determine the optimal sgRNAs for CRISPR-Cpf1 mutagenesis to exclude the ARE or introduce one of the six HPE SNVs.
- Converted_sgRNA2.0: Output from sgRNA Scorer 2.0 converted to comma separated files for faster file loading and use in Excel.
- Query_Sequence_ZIC2_3'UTR.fasta: the 1099 bp ZIC2 3'UTR sequence used for analysis in both Geneious and sgRNA Scorer 2.0
- Summary_csv_converter.R: R code used to convert the summary output file to .csv
- raw_output_from_sgRNA2.0_scorer.zip: Raw ouput from sgRNA Scorer 2.0