code: 1.Code for data preprocessing 2. Code for generating the phenotype file 3. Code for difference analysis with limma package 4. Code for GO and KEGG enrichment analysis 5. Code for Visualize example: exprSet.txt = data preprocessing output file phenotype.csv = generated phenotype file nrDEG_GSE10500.txt = difference analysis results GOenrich_GSE10500.txt = GO enrichment analysis results KEGGenrich_GSE10500.txt = KEGG enrichment analysis results