ctargon / TSPG

Transcriptome State Perturbation Generator

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tSNE Plot Wrong

ClearyM40 opened this issue · comments

I have been having an issue with how the tSNE plot is plotting the perturbation points on the graph. They are only partially moving towards the target class. Could there be an issue with how the perturbations are being calculated that's causing them to not fully reach the target class on the plot?
Attached are the output files, tSNE plot, and command lines used.
normal tsne (2)
Output_Files.txt
Commands.txt

@bentsherman @ctargon

Hi @ClearyM40 , I noticed this issue started to happen occasionally after I upgraded TSPG to Tensorflow 2.0. Unfortunately I was never able to figure out exactly why, and I no longer work for the lab that maintains this project, so I can only dig into the TSPG code every now and then.

What you can try is to run your analysis with TSPG v0.2 as that was the last commit before I upgraded to TF 2. If we can confirm that there is a notable difference, perhaps we can isolate the change that is messing things up.