Exclusion of reads with -F flag
aklein-705 opened this issue · comments
aklein-705 commented
I see the default for the F flag option has a default value of 1796. I was wondering if when excluding the PCR or optical duplicates, does mosdepth retain one of these duplicates that is then used in the coverage calculation? Or all all of them excluded? Thank you!
Brent Pedersen commented
mosdepth will exclude anything marked with the given flag(s). Usually, the software that mark duplicates will leave a single read or template that is not marked.
aklein-705 commented
Thanks so much for the quick reply!