biod / sambamba

Tools for working with SAM/BAM data

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sambamba markdup: Unable to write to stream

sklages opened this issue · comments

Well, running sambamba markdup results in an error when using --io-buffer-size=X with X being larger than 1024.
I read this in issue #169 and was not expecting it to be still an issue.

  • BAM: some BAMs, WGBS data, between 19GB and 35GB in size (created by bsmap)
  • sambamba: Version 0.8.0
  • params: --remove-duplicates --nthreads=24 --compression-level=4 --sort-buffer-size=8192 --io-buffer-size=X, X=1024 to 8192 in 1024 steps

Diskspace is plenty, RAM is 1TB, ulimit -n is 4096.

Any idea what I missed?

no response.