The Extensive de novo TE Annotator (EDTA)
Introduction
This package is developed for automated whole-genome de-novo TE annotation and benchmarking the annotation performance of TE libraries.
The EDTA package was designed to filter out false discoveries in raw TE candidates and generate a high-quality non-redundant TE library for whole-genome TE annotations. Selection of initial search programs were based on benckmarkings on the annotation performance using a manually curated TE library in the rice genome.
For benchmarking of a testing TE library, I have provided the curated TE annotation (v6.9.5) for the rice genome (TIGR7/MSU7 version). You may use the lib-test.pl
script to compare the annotation performance of your method/library to the methods we have tested (usage shown below).
Installation
conda create -n EDTA
conda activate EDTA
python2 -m pip install --user numpy==1.14.3 biopython==1.74 pp
conda config --env --add channels anaconda --add channels conda-forge --add channels biocore --add channels bioconda --add channels cyclus
conda install -n EDTA -y cd-hit repeatmodeler muscle mdust repeatmasker=4.0.9_p2 blast-legacy java-jdk perl perl-text-soundex multiprocess regex tensorflow=1.14.0 keras=2.2.4 scikit-learn=0.19.0 biopython pandas glob2 python=3.6 trf
git clone https://github.com/oushujun/EDTA
./EDTA/EDTA.pl
Alternative installation with Docker
More information: https://hub.docker.com/r/kapeel/edta
docker pull kapeel/edta
EDTA Usage
Activate the EDTA program:
conda activate EDTA
From head to toe
You got a genome and you want to get a high-quality TE library:
perl EDTA.pl [options]
-genome [File] The genome FASTA
-species [Rice|Maize|others] Specify the species for identification of TIR candidates. Default: others
-step [all|filter|final|anno] Specify which steps you want to run EDTA.
all: run the entire pipeline (default)
filter: start from raw TEs to the end.
final: start from filtered TEs to finalizing the run.
anno: perform whole-genome annotation/analysis after TE library construction.
-overwrite [0|1] If previous results are found, decide to overwrite (1, rerun) or not (0, default).
-cds [File] Provide a FASTA file containing the coding sequence (no introns, UTRs, nor TEs) of this genome or its close relative.
-curatedlib [file] Provided a curated library to keep consistant naming and classification for known TEs.
All TEs in this file will be trusted 100%, so please ONLY provide MANUALLY CURATED ones here.
This option is not mandatory. It's totally OK if no file is provided (default).
-sensitive [0|1] Use RepeatModeler to identify remaining TEs (1) or not (0, default).
This step is very slow and MAY help to recover some TEs.
-anno [0|1] Perform (1) or not perform (0, default) whole-genome TE annotation after TE library construction.
-evaluate [0|1] Evaluate (1) classification consistency of the TE annotation. (-anno 1 required). Default: 0.
This step is slow and does not affect the annotation result.
-exclude [File] Exclude bed format regions from TE annotation. Default: undef. (-anno 1 required).
-threads|-t [int] Number of theads to run this script (default: 4)
-help|-h Display this help info
Divide and conquer
Identify intact elements of a paticular TE type:
1.Get raw libraries from a genome (specify -type ltr|tir|helitron
in different runs)
perl EDTA_raw.pl [options]
-genome [File] The genome FASTA
-species [Rice|Maize|others] Specify the species for identification of TIR candidates. Default: others
-type [ltr|tir|helitron|all] Specify which type of raw TE candidates you want to get. Default: all
-overwrite [0|1] If previous results are found, decide to overwrite (1, rerun) or not (0, default).
-threads|-t [int] Number of theads to run this script
-help|-h Display this help info
2.Finish the rest of the EDTA analysis (specify -overwrite 0
and it will automatically pick up existing results in the work folder)
perl EDTA.pl -overwrite 0 [options]
Benchmarking
If you developed a new TE method/got a TE library and want to compare it's annotation performance to the methods we have tested, you can:
1.annotate the rice genome with your test library:
RepeatMasker -pa 36 -q -no_is -norna -nolow -div 40 -lib custom.TE.lib.fasta -cutoff 225 rice_genome.fasta
2.Test the annotation performance of a particular TE category.
perl lib-test.pl -genome genome.fasta -std genome.stdlib.RM.out -tst genome.testlib.RM.out -cat [options]
-genome [file] FASTA format genome sequence
-std [file] RepeatMasker .out file of the standard library
-tst [file] RepeatMasker .out file of the test library
-cat [string] Testing TE category. Use one of LTR|nonLTR|LINE|SINE|TIR|MITE|Helitron|Total|Classified
-N [0|1] Include Ns in total length of the genome. Defaule: 0 (not include Ns).
-unknown [0|1] Include unknown annotations to the testing category. This should be used when
the test library has no classification and you assume they all belong to the
target category specified by -cat. Default: 0 (not include unknowns)
eg.
perl lib-test.pl -genome rice_genome.fasta -std ./EDTA/database/Rice_MSU7.fasta.std6.9.5.out -tst rice_genome.fasta.test.out -cat LTR
Citation
Please cite our paper if you find EDTA is useful:
Ou S., Su W., Liao Y., Chougule K., Agda J. R. A., Hellinga A. J., Lugo C. S. B., Elliott T. A., Ware D., Peterson T., Jiang N.
Please also cite the software packages that were used in EDTA, listed in the EDTA/bin directory.
Other resources
You may download the rice genome here.
Issues
If you have any issues with installation and usage, please check if similar issues have been reported in Issues or open a new issue. If you are (looking for) happy users, please read or write successful cases here.