alexdobin / STAR

RNA-seq aligner

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Aligning reads to transcripts

sagnikbanerjee15 opened this issue · comments

Hello,

I have a set of reads sequenced on PacBio. I wish to align those to a transcriptomic reference that contains sequences highly similar to one another (some have only 3-6 nucleotides of difference). There is no genome so I cannot allow for a spliced alignment. I set the alignIntronMax and alignIntronMin to 0 but the final alignments still contain some splices. How do I turn it off entirely?
Also is there a way I can generate an expanded cigar with = and X?

Thanks.
Sagnik