hardclip reads from 5'
weitaiwu opened this issue · comments
hello. STAR is a good tool for alignment. But I met a problem when i use --clip5pNbases option.
I find it can only softclip my reads.
I want to clip 26bp(contain cellbarcode & UMI) from 5'.
How can I hardclip my reads from 5' using STARsolo options?