when I tried to generate the gene expression matrix, I can not generate .bam file with STAR 2.7.11b, 2.7.4a and 2.6.1d.

[ZChen90]

I am using STAR to generate the gene expression matrix. But the size of my .bam files is 0 and I checked the .err file. The .err file says as below:

$ cat SRR1039523.err

EXITING because of FATAL ERROR: Genome version: 2.7.1a is INCOMPATIBLE with running STAR version: 2.7.11b
SOLUTION: please re-generate genome from scratch with running version of STAR, or with version: 2.7.4a

So I removed STAR 2.7.11b and installed STAR 2.7.4a. Then I tried to generate the gene expression matrix again. But the size of .bam files is still 0. The .err file said :

EXITING because of FATAL ERROR: Genome version: 2.7.1a is INCOMPATIBLE with running STAR version: 2.7.4a
SOLUTION: please re-generate genome from scratch with running version of STAR, or with version: 2.7.4a

Then I tried STAR 2.6.1d. Then the .err file said:

EXITING because of FATAL ERROR: Genome version is INCOMPATIBLE with current STAR version
SOLUTION: please re-generate genome from scratch with the latest version of STAR

My computer has MacOS 14.2.1 (23C71). It is M1 chip. I downloaded the the index files: Human reference (GRCh38) - 2024-A, from 10x genomics (the link is https://www.10xgenomics.com/support/software/cell-ranger/downloads).

Could you please let me know how I can solve this problem? If you need more information, please let me know! Thanks a lot!

Chen

[Alexander Dobin]

Please generate the genome with the same STAR version that you use for mapping (i.e. 2.7.11b). This should solve all the problems.