alexdobin / STAR

RNA-seq aligner

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Issues when I try to use STAR to align RNAseq reads to genome index

jqzjqz0707 opened this issue · comments

I have an issue when I tried to use STAR to align RNAseq reads to genome index. I checked the process of making genome index. Here is my code " nohup STAR --runThreadN 4 --runMode genomeGenerate --genomeDir gindex --genomeFastaFiles polar.fna --genomeSAindexNbases 12 &", which I don't think cause the problem.

However, when I try to align RNAseq reads to genome index. (nohup STAR --runThreadN 4 --genomeDir /u02/qizhou/polar/gindex --readFilesIn *.fq --alignIntronMax 100000 --outSAMtype BAM SortedByCoordinate --outWigType wiggle --outWigStrand Unstranded &
). Here is the error: "/usr/bin/STAR: line 7: 1251459 Segmentation fault (core dumped) "${cmd}" "$@"".