We present HRIBO (High-throughput annotation by Ribo-seq), a workflow to enable reproducible and high-throughput analysis of bacterial Ribo-seq data. The workflow performs all required pre-processing steps and quality control. Importantly, HRIBO outputs annotation-independent ORF predictions based on two complementary prokaryotic-focused tools, and integrates them with additional computed features. This facilitates both the rapid discovery of ORFs and their prioritization for functional characterization.
RickGelhausen opened this issue · comments
"Distribution of aligned read lengths, to be sure they are mostly
between 26 and 34 nt, centred at 29-30 nt, and relatively even." - Sarah Svensson
"Read alignment stats - mapped reads, uniquely mapped, etc. to check
sequencing depth." - Sarah Svensson
"Reads mapping to RNA classes to check for rRNA/tRNA contamination." - Sarah Svennson
This is implemented.