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RickGelhausen / HRIBO

We present HRIBO (High-throughput annotation by Ribo-seq), a workflow to enable reproducible and high-throughput analysis of bacterial Ribo-seq data. The workflow performs all required pre-processing steps and quality control. Importantly, HRIBO outputs annotation-independent ORF predictions based on two complementary prokaryotic-focused tools, and integrates them with additional computed features. This facilitates both the rapid discovery of ORFs and their prioritization for functional characterization.

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mapping

RickGelhausen opened this issue · comments

commented
  • 3' end mapping
  • centroid mapping
  • 5' end mapping
commented

"Tracks for coverage with single-nucleotide mapping: 5' end of read,
3' end of read (first base and last base). Also centred approach (a la
https://www.nature.com/articles/nprot.2013.133). The last base
approach is usually the best and most widely used, so the most
important." - Sarah Svensson

commented

Functionality is available via READemption coverage,.. investigating

commented

Finalized with c69a7f5