QuanW / montage

Determining the most efficient tiling strategy for montage cryo-electron tomography

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montage

This package supports montage cryo-electron tomography (cryoET) as a technique to image a large field-of-view at high resolution.

The code in the collect folder enables scoring the efficiency of different tiling strategies by simulation and writing the desired strategy to an input file for SerialEM. Here we assume that data will be collected using a circular beam equipped with fringe-free illumination.

The process folder contains a series of scripts for assembling the acquired data into a montage tilt-series. Corrections during data pre-processing include Fresnel fringe removal, adjustment for uneven radial illumination, and CTF correction. Non-python dependencies include IMOD and CTFFIND4.

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Determining the most efficient tiling strategy for montage cryo-electron tomography


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