How to use Nextpolish on NextDenovo draft assembly
DrNavi opened this issue · comments
Hi, I am Dr. Naveed, i am new to perform Genome Assembly. I have nanopore long read data and I successfully used NextDenovo to draft a fungus assembly. I have got nd.asm.fasta file after assembly from NextDenovo, 11 contigs are generated. Now, I wanted to polish my draft assembly using NextPolish tool. What command I can use for polishing because when I saw the tutorial, I found this command is used to make input file for long read polishing,
ls reads1.fq reads2.fa.gz > lgs.fofn
However, I just got one output file from NextDenovo, which I want to polish.....
I hope I made my question clear here..... I need guidance
reads1.fq reads2.fa.gz are the nanopore long read data files, same input as NextDenovo
Dear Moold,
I only get only sequencing file from the company, "KI.fasta.tar.gz