ERROR：the input data is insufficient for an assembly
Argonum-Clever2 opened this issue · comments
Error message
*Suggested seed_cutoff (genome size: 680.00Mb, expected seed depth: 45, real seed depth: 9.80): 50001 bp
[113064 ERROR] 2023-11-21 10:04:54 the input data is insufficient for an assembly.
Config file
[General]
job_type = local # local, slurm, sge, pbs, lsf
job_prefix = nextDenovo
task = all # all, correct, assemble
rewrite = yes # yes/no
deltmp = yes
parallel_jobs = 20 # number of tasks used to run in parallel
input_type = raw # raw, corrected
read_type = ont # clr, ont, hifi
input_fofn = ej.fofn
workdir = /data0/stu_wangfang/software/NextDenovo
[correct_option]
genome_size = 680m # estimated genome size
sort_options = -m 20g -t 15
minimap2_options_raw = -t 8
pa_correction = 3 # number of corrected tasks used to run in parallel, each correct
correction_options = -p 15
[assemble_option]
minimap2_options_cns = -t 8
nextgraph_options = -a 1
Operating system
LSB Version: :core-4.1-amd64:core-4.1-noarch
Distributor ID: CentOS
Description: CentOS Linux release 7.9.2009 (Core)
Release: 7.9.2009
Codename: Core
GCC
gcc version 4.8.5 20150623 (Red Hat 4.8.5-44) (GCC)
Python
Python 3.9.12
As the log says, the input data is insufficient for an assembly, so please input more data.