NAMlab / variant-caller

Variant Calling Pipeline Using GATK4 and Nextflow

variant-caller

Pipeline to call SNPs and indels from sequencing reads and a reference genome, based on the variant-calling-pipeline-gatk4 from gencorefacility (https://github.com/gencorefacility/variant-calling-pipeline-gatk4, usage tutorial: https://gencore.bio.nyu.edu/variant-calling-pipeline-gatk4/).

Changes:

• natively use Singularity instead of Docker (https://cloud.sylabs.io/library/merlin/default/namlab-variant-caller)
• start from a list of SRA accessions instead of fastq files
• add trimmomatic
• use bcftools/csq instead of snpEff