Improved_Seurat_Pre_Process String Idents
alperoglu opened this issue · comments
I suppose this is a minor issue that can be resolved simple data adjustments to the Seurat Object input. I am writing this as a future reference to other people that have the same issue.
The FindMarkers function uses 0 for the ident.1 argument and this causes issues with string identifiers. It gives the following error when the input cluster identifiers are strings:
Error in WhichCells.Seurat: Cannot find the following identities in the object: 0
I was using the output data from this Seurat tutorial : https://satijalab.org/seurat/v3.1/pbmc3k_tutorial.html
I changed the string identifiers to the seurat_cluster identifiers in the metadata that were the numeric equivalents of the cell type names.